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Promega glucose uptake-glotm assay
Glucose Uptake Glotm Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glucose+uptake-glotm+assay/glucose+uptake+glo+assay/pmc10014232__mmc5-269-6-9
Average 90 stars, based on 1 article reviews
glucose uptake-glotm assay - by Bioz Stars, 2026-10
90/100 stars

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cDNA Synthesis:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Real-time Polymerase Chain Reaction:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

SYBR Green Assay:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

RNA Sequencing:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Construct:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Recombinant:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Plasmid Preparation:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Software:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Functional Assay:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Flow Cytometry:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Control:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Lysis:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Activation Assay:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Transfection:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Luciferase:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Viability Assay:


Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

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Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Cellular glucose uptake was analyzed using Glucose Uptake-GloTM Assay (Promega) as per manufacturer’s instructions.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: Figure S4. metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state
Article Snippet: Glucose Uptake-GloTM Assay , Promega , CatJ1341.

Article Title: Combined thioredoxin reductase and glutaminase inhibition exerts synergistic anti-tumor activity in MYC-high high-grade serous ovarian carcinoma
Article Snippet: 10 Cellular glucose uptake in auranofin-treated MYC-high HGSOC cells and glycolysis metabolites levels in auranofin-treated MYC-low PEO4 cells. (A) MYC-high OVCAR-8 (Blue) and PEO1 (Red) cells were treated with or without auranofin (2.5 μM) for 24 h and intracellular glucose uptake was analysed by Glucose Uptake-GloTM Assay (Promega). (B) PEO4 cells were treated either with DMSO or 2.5 μM auranofin for 24 h. Cells were then subjected to targeted Central Carbon Metabolomics using LC/MS-MS. Intracellular concentrations of key glycolytic metabolites, both upstream and downstream of GAPDH, are shown.



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